雞禽流感病毒(AIV)ELISA試劑盒常見(jiàn)問(wèn)題解答：高背景的原因是什么？答：1）洗滌不當(dāng)：檢查洗滌緩沖罐的容量，確保按說(shuō)明書操作進(jìn)行洗滌步驟。2）被污染的儀器：使用前確保基材沒(méi)有被金屬離子或氧化劑污染。在使用底物溶液前確保單獨(dú)保存額外的底物溶液。3）酶標(biāo)板暴露于光：可能導(dǎo)致酶標(biāo)板呈藍(lán)色。4）錯(cuò)誤的溫育時(shí)間/溫度：通常遵循標(biāo)準(zhǔn)的溫育時(shí)間和溫度。如果在標(biāo)準(zhǔn)稀釋系列中沒(méi)有觀察到強(qiáng)梯度的情況下，所有的孔都是強(qiáng)烈且均勻的顏色，那么可能需要在顏色顯影時(shí)觀察基質(zhì)反應(yīng)，以便更快的停止反應(yīng)。Q:What are the reasons for High Background?A:1) Improper Washing: Check volume of washing buffer reservoir and make sure all recommended washing steps are performed. 2) Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 3) Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate. 4) Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times and temperatures. However, if all wells are intensely and equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

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